In my recent blog DCIS and DNA Cytophtometry I referred to a publication by Emson and Kirk who used this technique on intraductal carcinoma of the breast. Their article appeared in Cancer 20: 1248. 1967.
Because I was employed from 1961-1965 as a cytologist at the Department of Cancer Research in Saskatoon, had constructed a microspectrophotometer did DNA Cytophotometry, also had trained the technician Brian Kirk to perform DNA measurements on this equipment, I was curious to know the methology that these investigators used.
To my knowledge since this was the only microspectrophotometer present at the university of Saskatchewan when the investigators submitted their manuscript for publication in 1966, I assumed their research was done using this equipment and that Brian Kirk did the Feulgen staining, measurements and calculations of DNA values,etc. In their section on material and methods, the authors did not have any such acknowledgements referenced to me, for the equipment and/or for the technical training. Additionally maybe before submitting their manuscript for publication the authors might have consulted with me to evaluate their data.
Incidentally after leaving the Department of Cancer Research in June 1965 I moved to Winnipeg to help establish the Winnipeg Clinic Research Institute Laboratory where I became its first medical director. One of the research projects at this private laboratory was to use DNA cytophotometry as a screening test for cervical smears using the commercial micro photometer (Reichert) . Some of this research was published in Acta Cytol. in 1969.
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